Epitope tagging is a technique that employs genetic engineering to fuse a known epitope, called an affinity tag, to either the C or N terminus of a recombinant protein to facilitate affinity ...

Creating analytical tools that support the advancement of knowledge can often come about through the integration of existing information and techniques, theoretical and practical, directed toward a ...

Affinity chromatography theoretically does just that—a ligand that specifically interacts with the target protein is immobilized on a chromatography matrix; the target protein binds to the column, and ...

Protein purification can be a challenging process. Since proteins are expressed in biological systems such as cells, the protein must be purified from a complex mixture. Generic column-based ...

The present purification protocol applies to target proteins that are fused to a double tag, such as NusA–His 6, through a linker that includes a protease-recognition sequence. It involves two steps ...

A revolutionary purification resin for C-terminal EPEA tagged proteins combining highly selective binding, even under denaturing condition, with mild elution of the target using the shortest affinity ...

Self-removing affinity tags have the potential to greatly accelerate the development of new therapeutics, while providing a potentially enabling manufacturing platform for non-mAb therapeutic proteins ...

Although affinity tag methods for protein purification have greatly accelerated research in laboratories throughout the world, the presence of the tag on the purified target can modify the target and ...